Glycoprotein Ib and glycoprotein IX are fully complexed in the intact platelet membrane.

摘要: Two new murine monoclonal antibodies, AK 1 and SZ 1, reactive with the human platelet glycoprotein (GP) Ib-IX complex have been produced by hybridoma technique. Both immunoprecipitated GP from Triton X-100-solubilized, periodate-labeled platelets. With trypsinized, labeled platelets, FMC 25 (epitope on IX) a membrane-bound proteolytic fragment of consisting IX an congruent to 25,000 mol wt remnant alpha-chain lb disulfide-linked beta-subunit. Unexpectedly, although purified complex, neither antibody individual components this Ib or IX. When were recombined, however, again reformed strongly suggesting that both antibodies recognizing epitope present only intact complex. Cross-blocking studies indicated recognized very similar identical was proximal for 25. bound number binding sites (congruent 25,000) platelets as directed against either The combined data suggests are fully complexed in membrane.