MicroRNA-26a targets the histone methyltransferase Enhancer of Zeste homolog 2 during myogenesis.

摘要: MicroRNA (miRNA) are important regulators of many bio×logical processes, but the targets for most miRNA still poorly defined. In this study, we profiled expression during myogenesis, from proliferating myoblasts through to terminally differentiated myotubes. Microarray results identified six significantly differentially expressed that were more than 2-fold different in From list, miRNA-26a (miR-26a), an up-regulated miRNA, was further examined. Overexpression miR-26a murine myogenic C2C12 cells induced creatine kinase activity, enzyme markedly increases myogenesis. Further, myoD and myogenin mRNA levels also up-regulated. These suggest increased promotes Through a bioinformatics approach, histone methyltransferase, Enhancer Zeste homolog 2 (Ezh2), as potential target miR-26a. suppressed activity luciferase reporter construct fused with 3′-untranslated region Ezh2. addition, overexpression decreased Ezh2 expression. reveal model regulation myogenesis whereby up-regulation acts post-transcriptionally repress Ezh2, known suppressor skeletal muscle cell differentiation.