Detection of Airborne Inoculum of Hymenoscyphus fraxineus and H. albidus during Seasonal Fluctuations Associated with Absence of Apothecia

作者: Milon Dvorak , Gabriela Rotkova , Leticia Botella

DOI: 10.3390/F7010001

关键词: Hymenoscyphus albidusBiological dispersalLeaf wetnessAscocarpBiologySporeFraxinusHorticultureBotanyHymenoscyphus fraxineusFungus

摘要: Hymenoscyphus fraxineus is an invasive fungal species causing the most serious disease of ashes (Fraxinus spp.) in Europe—ash dieback. The biology this fungus not totally elucidated, neither its relation to saprophytic albidus, native Europe. Our study focused on description seasonal spore dispersal both fungi and meteorological conditions, which needed for more precise effective control disease. For experiment one long time infected mixed forest SE Czech Republic was chosen. A seven-day automatic volumetric trap a weather station were installed continuously sample aerospora from April October 2014. In seven periods rotating arm also used obtain 48-h air samples compare efficiency these two types samplers. Air evaluated solely by qPCR with very low detection limit. Results show co-occurrence inoculum throughout entire sampling period peak levels August. origin sampled without apothecia discussed. Air-inoculum occurrence significantly correlated each other, suggesting their coexistence forest.

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