Detection of Legionella species in sewage and ocean water by polymerase chain reaction, direct fluorescent-antibody, and plate culture methods.

摘要: Legionella spp. are ubiquitous in most environmental water sources; however, sewage treatment plants have not been examined as potential reservoirs for these bacteria. This study used polymerase chain reaction, direct fluorescent-antibody staining, and culture methods to examine raw treated sewage, ocean-receiving waters, nearshore coastal environments the presence of pneumophila other The concluded that present all phases population numbers do significantly decline through process. Ocean-receiving waters located 5 miles offshore, where is discharged, were found contain spp., but ocean between discharge site bathing beaches was negative. suggests from reaching beach waters. A flood control channel river entered a nearby swimming area also be positive, suggesting land runoff source samples tested positive.