Legionella pneumophilla bacteria in a thermal saline bath

作者: Maciej Walczak , Arkadiusz Krawiec , Elżbieta Lalke-Porczyk

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摘要: OBJECTIVE The study was aimed at determining whether Legionella pneumophila bacteria can be found in thermal saline waters used balneotherapy. METHODS Water samples were collected from three baths, supplied by (type Cl - Na). total number of determined the direct microscopic count under a fluorescence microscope numbers belonging to different phylogenetic groups (Eubacteria, sp. and pneumophila) with use molecular FISH method. RESULTS highest average as well Eubacteria entire research cycle recorded bath 1. Bacteria genus along identified all water each bath. Moreover, biofilm containing cells L. pneumophilla samples. CONCLUSION increases bath's age. successfully develop not only fresh bodies but baths well. Still, it is uncertain commonly applied culture method, developed for freshwater bodies, also suitable baths.

参考文章(16)
C J Palmer, Y L Tsai, C Paszko-Kolva, C Mayer, L R Sangermano, Detection of Legionella species in sewage and ocean water by polymerase chain reaction, direct fluorescent-antibody, and plate culture methods. Applied and Environmental Microbiology. ,vol. 59, pp. 3618- 3624 ,(1993) , 10.1128/AEM.59.11.3618-3624.1993
Martin Exner, Philippe Hartemann, Summary of the second meeting of the International Forum on Water Hygiene in Buildings (IFOWAHB) from 01 to 02.06.2007 in Stockholm. International Journal of Hygiene and Environmental Health. ,vol. 212, pp. 449- 458 ,(2009) , 10.1016/J.IJHEH.2008.12.001
Priscilla Declerck, Biofilms: the environmental playground of Legionella pneumophila Environmental Microbiology. ,vol. 12, pp. 557- 566 ,(2010) , 10.1111/J.1462-2920.2009.02025.X
Cyril Guyard, Donald E. Low, Legionella infections and travel associated legionellosis Travel Medicine and Infectious Disease. ,vol. 9, pp. 176- 186 ,(2011) , 10.1016/J.TMAID.2010.05.006
Priscilla Declerck, Jonas Behets, Vincent van Hoef, Frans Ollevier, Detection of Legionella spp. and some of their amoeba hosts in floating biofilms from anthropogenic and natural aquatic environments Water Research. ,vol. 41, pp. 3159- 3167 ,(2007) , 10.1016/J.WATRES.2007.04.011
Klaus Heuner, Michael Steinert, The flagellum of Legionella pneumophila and its link to the expression of the virulent phenotype International Journal of Medical Microbiology. ,vol. 293, pp. 133- 143 ,(2003) , 10.1078/1438-4221-00259
James M. Barbaree, Anthony Sanchez, Gary N. Sanden, Tolerance of Legionella species to sodium chloride Current Microbiology. ,vol. 9, pp. 1- 5 ,(1983) , 10.1007/BF01567125
Dorothee Grimm, Wolfgang Ludwig, Bettina Brandt, Rolf Michel, Karl-Heinz Schleifer, Jörg Hacker, Michael Steinert, Development of 18S rRNA-targeted Oligonucleotide Probes for Specific Detection of Hartmannella and Naegleria in Legionella – positive Environmental Samples Systematic and Applied Microbiology. ,vol. 24, pp. 76- 82 ,(2001) , 10.1078/0723-2020-00017
Shih-Wei Huang, Bing-Mu Hsu, Shu-Fen Wu, Cheng-Wei Fan, Feng-Cheng Shih, Yung-Chang Lin, Dar-Der Ji, Water quality parameters associated with prevalence of Legionella in hot spring facility water bodies. Water Research. ,vol. 44, pp. 4805- 4811 ,(2010) , 10.1016/J.WATRES.2010.07.063
W. J. Patterson, D. V. Seal, E. Curran, T. M. Sinclair, J. C. McLuckie, Fatal nosocomial Legionnaires' disease: relevance of contamination of hospital water supply by temperature-dependent buoyancy-driven flow from spur pipes. Epidemiology and Infection. ,vol. 112, pp. 513- 525 ,(1994) , 10.1017/S0950268800051219