An ELISA for the detection of anti-neutrophil cytoplasm antibodies (ANCA).
作者： Niels Rasmussen , Carola Sjölin , Bengt Isaksson , Per Bygren , Jörgen Wieslander
DOI: 10.1016/0022-1759(90)90350-5
关键词: Antibody 、 Staining 、 Chemistry 、 Primary and secondary antibodies 、 Percoll gradient centrifugation 、 Pathology 、 ANCA POSITIVE 、 Cytoplasm 、 Molecular biology 、 Indirect immunofluorescence 、 IIf
摘要: Abstract An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of circulating anti-neutrophil cytoplasm antibodies (ANCA), which are defined by a diffuse, granular staining alcohol-fixed human neutrophils indirect immunofluorescence (IIF). Detection high sensitivity and specificity active Wegener's granulomatosis (WG) reflects effect treatment. In present assay, immunoplates were coated with cytoplasmic α fraction obtained from apparently healthy donors nitrogen bomb cavitation subsequent Percoll gradient centrifugation. Alkaline phosphatase-labelled anti-human IgG was used as secondary antibody. Diluted sera 70 patients WG 16 other diseases anti-myeloperoxidase (anti-MPO) examined. It is concluded that ELISA accurately detects IIF ANCA positive WG, helpful in detecting remission, not influenced presence anti-MPO may help cases granulocyte-specific anti-nuclear since this pattern obscures patterns. The titration can be carried out 3.5 h whereas rapid test just to detect performed 30 min.
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