Modulation of a Voltage-activated Potassium Channel by Peptide Growth Factor Receptors

摘要: Regulation of the activity a cloned component voltage-activated K+ channel, Kv1.5, was studied by expressing channel and receptors for platelet-derived growth factor (PDGF) or fibroblast (FGF) simultaneously in Xenopus oocytes. Receptor activation mediated decline Kv1.5 current amplitude, with half-time about 20 min. The reduction amplitude occurred little change kinetics voltage sensitivity activation. A similar phenomenon found when human thrombin rat 5-HT1c receptors, two that increase phospholipase C activity, were tested coexpression experiments. mutant FGF receptor, which does not activate C-gamma 1 but retains several its other functions, did modulate current. Simultaneous injection inositol trisphosphate superfusion phorbol 12-myristate 13-acetate reproduced modulation These results demonstrate PDGF can increasing suggest may be able to alter rapidly electrical excitability neurons.