Role of miR-145 in chronic constriction injury in rats.
作者： Xiaolin Pang , Yuanzhang Tang , Dongya Zhang
关键词: Kinase 、 Internal medicine 、 Spinal cord 、 Messenger RNA 、 Oncogene 、 Blot 、 Pathology 、 Gene knockdown 、 Biology 、 Endocrinology 、 Apoptosis 、 Molecular medicine
摘要: The present study aims to investigate the effects and underlying mechanisms of miRNA-145 (miR-145) in rat models chronic constriction injury (CCI). Rats were randomly divided into control, sham, CCI, agomiRNA (agomiR)-normal control (NC) agomiR-145 groups (n=25 each group); addition, 30 rats with CCI small hairpin (sh)RNA-NC shRNA-ras responsive element binding protein 1 (RREB1) groups. Paw withdrawal thermal latency (PWTL) paw mechanical threshold (PWMT) detected. Reverse transcription-quantitative polymerase chain reaction was used detect miR-145 expression levels, western blotting performed measure RREB1 phosphorylated-protein kinase B (p-AKT) levels. In a dual luciferase reporter assay conducted identify target gene miR-145. PWMT PWTL decreased this decrease alleviated by injection. At 1, 3, 5 7 days after level spinal cord tissue group significantly compared day before (P<0.05). Compared group, higher levels p-AKT increased Furthermore, knockdown shRNA-RREB1 values PWTL, p-AKT, Further investigation demonstrated that can bind mRNA. conclusion, may be involved development through regulating RREB1.
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