Insights into the achaete-scute homolog-1 gene (hASH1) in normal and neoplastic human lung.

摘要: Achaete-scute homolog-1 (ASH1) is pivotal for the development of pulmonary neuroendocrine (NE) cells. We examined human ASH1 (hASH1) expression across a comprehensive panel lung cancer cell lines, primary tumors and normal fetal post-natal lungs. While hASH1 was cardinal feature NE carcinomas, subgroup non-NE cancers also exhibited this factor. Twenty lines out 33 were positive mRNA by reverse transcription PCR, including 6/6 small carcinomas (SCLC), 5/5 carcinoids, 6/7 non-SCLC with features, 3/14 other non-SCLC. Among tumors, 2/2 SCLC, 10/41 (only 4 which had features) immunohistochemistry RNA-RNA in situ hybridization. In lung, neural marker synaptophysin highly concordant neuroepithelial bodies solitary cells, while rest epithelium negative. childhood adulthood, markers became progressively discordant, majority hASH1-immunoreactive foci (69%) being negative adults, potentially representing dormant progenitors. conclude that provides an early indication program lung.