Role of malate dehydrogenase in facilitating lactate dehydrogenase to support the glycolysis pathway in tumors

摘要: High aerobic glycolysis, as one of the hallmarks cancer cells, requires nicotinamide adenine dinucleotide (NAD+) a vital co-factor, to guarantee flow glycolysis. Malate dehydrogenase (MDH), an important enzyme in metabolism, is source NAD+ additional lactate (LDH). The current study aimed elucidate kinetic parameters MDH human breast and evaluate its supportive role glycolysis pathway. Michaelis-Menten constant (Km) maximum velocity (Vmax) were determined crude extracts tumors healthy tissue samples, which obtained directly from operating theatre. To assess potential supporting activity was measured when LDH inhibited by different concentrations oxamate, inhibitor cell lines. Km cancerous (C-MDH) same MDH, although Vmax C-MDH higher relative MDH. Notably, increased MDA-MB-231 line, treated with (oxamate), but not MCF-7 line (P<0.05). tendency for malate generation cells effective approach Increasing absence demonstrates Therefore, decreasing expression forward reaction may present valid molecular target abolish effect on tumor metabolism.