Dual-labeled oligonucleotide probe for sensing adenosine via FRET: a novel alternative to SNPs genotyping.
作者： Yoshio Saito , Subhendu Sekhar Bag , Yuichi Kusakabe , Chiharu Nagai , Katsuhiko Matsumoto
DOI: 10.1039/B618465K
关键词: Sequence analysis 、 Sequence (biology) 、 Biochemistry 、 Biophysics 、 Adenosine 、 Oligomer restriction 、 Genotyping 、 Nucleoside 、 Chemistry 、 Fluorescence 、 Förster resonance energy transfer
摘要: A novel FRET based strategy for DNA sequence analysis utilising base-discriminating fluorescence (BDF) nucleoside, PyU/2-AntU, as donor in the dual-labelled oligonucleotide probe is reported; a selective/specific emission from acceptor, was observed upon excitation at donor, only when opposite base of “smart” fluorescently labeled BDF adenine on complementary target sequence.
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