Locked nucleic acid: a potent nucleic acid analog in therapeutics and biotechnology.
作者： Jan Stenvang Jepsen , Mads D. Sørensen , Jesper Wengel
关键词: In vitro 、 Locked nucleic acid 、 Oligonucleotide 、 Regulation of gene expression 、 Nucleic acid 、 Biology 、 Biochemistry 、 RNA 、 Complementary DNA 、 RNase H
摘要: Locked nucleic acid (LNA) is a class of analogs possessing very high affinity and excellent specificity toward complementary DNA RNA, LNA oligonucleotides have been applied as antisense molecules both in vitro vivo. In this review, we briefly describe the basic physiochemical properties some difficulties that may be encountered when applying technology. The central part review focuses on use regulation gene expression, including delivery to cells, stability, unspecific effects, toxicity, pharmacokinetics, design oligonucleotides. last evaluates diagnostic tool genotyping.
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