GROWTH OF HUMAN NORMAL ERYTHROID PROGENITORS IN LIQUID CULTURE : A COMPARISON WITH COLONY GROWTH IN SEMISOLID CULTURE
作者： E. Fibach , D. Manor , A. Treves , E. A. Rachmilewitz
关键词: Cell biology 、 Immunology 、 Cell culture 、 Stem cell 、 Erythropoiesis 、 Cell division 、 Erythropoietin 、 Biology 、 Erythroid Precursor Cells 、 Population 、 Cell growth
摘要: Most studies of erythropoiesis in vitro have employed cloning methods semisolid medium. We recently described a two-step liquid culture procedure that supports the proliferation and differentiation human erythroid progenitors. In present study, we modified to allow large-scale cultures cells derived from normal donors. The is divided into two phases. first phase, which erythropoietin (Epo) independent, early progenitors multiply differentiate. second, Epo-dependent they mature orthochromatic normoblasts enucleated erythrocytes. Using this procedure, cell yield reached 7.5 x 10(6)/ml total 7 10(8) could be harvested per blood unit. A comparison growth their colony indicated was superior: 1) terms originally cultured mononuclear cell, 2) ml surface area purity resultant population. addition, it permits easier manipulation condition components sampling greater than 1 10(7) at each maturation stage subsequent proerythroblast stage. This might provide an important experimental tool for studying development.
sci-hub.se 参考文章(11)
J. F. Borel, B. Ryffel, The Mechanism of Action of Ciclosporin: A Continuing Puzzle Springer, Berlin, Heidelberg. pp. 24- 32 ,(1985) , 10.1007/978-3-642-70607-3_4
G Hale, S Bright, G Chumbley, T Hoang, D Metcalf, AJ Munro, H Waldmann, Removal of T cells from bone marrow for transplantation: a monoclonal antilymphocyte antibody that fixes human complement Blood. ,vol. 62, pp. 873- 882 ,(1983) , 10.1182/BLOOD.V62.4.873.873
CB Lozzio, BB Lozzio, Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood. ,vol. 45, pp. 321- 334 ,(1975) , 10.1182/BLOOD.V45.3.321.321
CD Myers, FE Katz, G Joshi, JL Millar, A Cell Line Secreting Stimulating Factors for CFU-GEMM Culture Blood. ,vol. 64, pp. 152- 155 ,(1984) , 10.1182/BLOOD.V64.1.152.152
M Ogawa, OC Grush, RF O'Dell, H Hara, MD MacEachern, Circulating Erythropoietic Precursors Assessed in Culture: Characterization in Normal Men and Patients With Hemoglobinopathies Blood. ,vol. 50, pp. 1081- 1092 ,(1977) , 10.1182/BLOOD.V50.6.1081.1081
E Fibach, D Manor, A Oppenheim, EA Rachmilewitz, Proliferation and maturation of human erythroid progenitors in liquid culture. Blood. ,vol. 73, pp. 100- 103 ,(1989) , 10.1182/BLOOD.V73.1.100.100
C. Friend, W. Scher, J. G. Holland, T. Sato, Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 68, pp. 378- 382 ,(1971) , 10.1073/PNAS.68.2.378
B. J. Clarke, D. Housman, Characterization of an erythroid precursor cell of high proliferative capacity in normal human peripheral blood. Proceedings of the National Academy of Sciences. ,vol. 74, pp. 1105- 1109 ,(1977) , 10.1073/PNAS.74.3.1105
J. R. Stephenson, A. A. Axelrad, D. L. McLeod, M. M. Shreeve, Induction of colonies of hemoglobin-synthesizing cells by erythropoietin in vitro. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 68, pp. 1542- 1546 ,(1971) , 10.1073/PNAS.68.7.1542
S. H. Orkin, F. I. Harosi, P. Leder, Differentiation in erythroleukemic cells and their somatic hybrids. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 72, pp. 98- 102 ,(1975) , 10.1073/PNAS.72.1.98