The metabolic demands of cancer cells are coupled to their size and protein synthesis rates

摘要: Although cells require nutrients to proliferate, most nutrient exchange rates of the NCI60 panel cancer cell lines correlate poorly with their proliferation rate. Here, we provide evidence indicating that this inconsistency is rooted in variability size. We integrate previously reported data characterizing genome copy number variations, gene expression, protein expression and fluxes our own measurements size content lines. show content, DNA synthesis per are proportional volume, larger proliferate slower than smaller cells. estimate metabolic these magnitudes rate and, after correcting for At level observe genes expressed at higher levels enriched involved cycle, while large mesenchymal The latter finding further corroborated by induction those same following treatment TGFβ, high vimentin but low E-cadherin also find aromatase inhibitors, statins mTOR inhibitors preferentially inhibit vitro growth cell. display various activities, type activity they possess correlates volume content. In addition proliferation, and/or biomarkers may predict response drugs targeting metabolism.