Progesterone “Receptor” in Rabbit Uterus.I. Characterization and Estradiol–17β Augmentation

摘要: Sucrose gradient centrifugation, gel nitration, and charcoal adsorption techniques have been used successfully to isolate partially characterize progesterone—binding proteins from rabbit uterine cytosol nuclei. Dissociability in high ionic strength buffers precipitability 30% saturated ammonium sulfate differentiated the binding component a CBG—like protein present serum. Progesterone was bound 4S complex following injection of 3H—progesterone castrate rabbits; hormone increased quantitatively after pretreatment animals with estradiol– 17β qualitative change pattern became apparent appearance an 8S addition unit. 0.4M KC1 extraction nuclear pellet obtained estrogen—treated castrates yielded only component. Rabbit prepared either or vitro, when equilibrated 3Hprogeste...