Molecular genetic detection of Bacteroides heparinolyticus in adult periodontitis

摘要: Bacteroides heparinolyticus in subgingival plaque was identified using a digoxigenin-labeled whole genomic DNA probe and polymerase chain reaction (PCR) assay based on 16S rRNA species-specific primers (5'-ATG GTG ATT CCG CAT GGT TTC TCC-3' (base position, 188-212) 5'-CAA ACT ACA GCT GAC TTA AGC-3' (592-615)). Subgingival specimens obtained by paper points from 3 deep periodontal pockets each of 113 adults were examined. The reacted with all pure isolates tested B. did not react other oral species tested; the showed positive reactions 74.3% patient samples PCR produced 428 bp specific amplification product test strains reveal detectable amplicons species. method detected 50 cells dispersed plaque. only revealed 6.2% studied. higher level probably due to false-positive cross-hybridization unknown This study suggests that amplifying sequences represents an easy valuable means detect low prevalence does incriminate organism etiology adult periodontitis.