Development of a serum-free medium for growth of NS-1 mouse myeloma cells and its application to the isolation of NS-1 hybridomas.
作者： Tomoyuki Kawamoto , J.Denry Sato , Anh Le , Don B. McClure , Gordon H. Sato
DOI: 10.1016/0003-2697(83)90614-0
关键词: Multiple myeloma 、 Molecular biology 、 Cell division 、 Chemistry 、 Serum free medium 、 Isolation (microbiology) 、 Spleen 、 Antibody 、 Cell fusion 、 Cell culture
摘要: In this study, we have devised a hormone-supplemented, lipid-enriched serum-free medium, designated KSLM, that supports the growth of NS-1 mouse myeloma cells and studied its applicability to efficient isolation antibody-producing hybridomas formed from fusion spleen immunized mice. Our results show KSLM when used in conjunction with our hybridization protocol, allowed for isolation, reproducible manner, antibody-secreting hybridomas. Moreover, yield was similar medium serum-supplemented medium. Here, also report on adaptation lipid-deficient The use adapted (NS-1-503), instead cells, experiments not only permitted lipid-free but resulted higher both complete
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