IL-12 regulation of parasite antigen-driven IgE production in human helminth infections.

摘要: Recombinant IL-12 inhibits IgE synthesis by IL-4-stimulated lymphocytes from healthy persons and influences the development of Th subset selection involved in Ig isotype selection. Whether endogenous production modulates patients with elevated serum is unknown. To examine this question we studied effects neutralizing anti-IL-12 or recombinant on parasite Ag-driven polyclonal corresponding IFN-gamma IL-4 PBMC helminth-infected individuals. The addition Ab significantly inhibited 11 12 individuals (p < 0.001). (1-10 U/ml) suppressed a dose-dependent fashion up to 94% relative untreated cultures. effect occurred, part, suppressing Ag-induced augmenting production. did not suppress purified B cells. An IFN-gamma-independent was suggested ability human rIL-12 presence anti-IFN-gamma. This study demonstrates that helminth production, regulating quantities generated Ag-specific lymphocytes.