Mucin (MUC) Gene Expression in Human Pancreatic Adenocarcinoma and Chronic Pancreatitis: A Potential Role of MUC4 as a Tumor Marker of Diagnostic Significance

摘要: Purpose: Mucins are important biomolecules that frequently display an altered expression under pathological conditions. In a search for unique and reliable marker(s) specific pancreatic adenocarcinoma, we investigated the of different MUC genes in tumors tumor cell lines, chronic pancreatitis, normal pancreas. Experimental Design: Total RNA from 16 tumors, 10 pancreatitis tissues, 7 pancreas 15 lines were analyzed by reverse transcription-PCR with primers MUC1 , MUC2 MUC3 MUC4 MUC5AC MUC5B MUC6 MUC7 slot blot analyses. Results: Our results revealed all mucins examined, only displayed differential was adenocarcinoma. Indeed, substantial number tissue samples (12 16) (11 15) expressed mRNA, whereas (0 10) 7) tissues failed to exhibit any detectable level this mucin. contrast, no significant alteration observed other relative samples. Conclusions: Overall, work demonstrates mucin is tumor-associated Furthermore, present study introduces novel avenue discriminate between adenocarcinoma pancreatitis. Future investigations role played may prove be useful formulation strategies diagnosis therapeutic treatment malignancy.