A bacterial protein promotes the recognition of the Legionella pneumophila vacuole by autophagy.

摘要: Legionella pneumophila (L. pneumophila) is an intracellular bacterium of human alveolar macrophages that causes Legionnaires' disease. In contrast to humans, most inbred mouse strains are restrictive L. replication. We demonstrate autophagy targets vacuoles lysosomes and this process requires ubiquitination the subsequent binding autophagic adaptor p62/SQSTM1 ubiquitinated vacuoles. The legA9 encodes for ankyrin-containing protein with unknown role. show mutant replicate in WT mice their bone marrow-derived macrophages. This first be found other than Fla mutant. Less mutant-containing acquired ubiquitin labeling staining, evading uptake avoiding lysosomal fusion. Thus, we describe a bacterial pneumophila-containing vacuole uptake.