The ubiquitin-binding adaptor proteins p62/SQSTM1 and NDP52 are recruited independently to bacteria-associated microdomains to target Salmonella to the autophagy pathway

摘要: Autophagy is an innate immune defense against bacterial invasion. Recent studies show that two adaptor proteins, p62 and NDP52, are required for autophagy of the pathogen Salmonella enterica serovar Typhimurium (S. typhimurium). However, it not known why different adaptors to target same cargo autophagy. Here we both recruited bacteria with similar kinetics, they independently each other, depletion either leads impairment antibacterial Depletion does synergistically impair autophagy, indicating act in pathway. Remarkably, observed these do colocalize, but rather form non-overlapping microdomains surrounding bacteria. We conclude NDP52 cooperatively drive efficient by targeting protein complexes coordinate distinct micro-domains associated