COLD-PCR enhanced melting curve analysis improves diagnostic accuracy for KRAS mutations in colorectal carcinoma
作者： Colin C Pritchard , Laura Akagi , Poluru L Reddy , Loren Joseph , Jonathan F Tait
关键词: Bioinformatics 、 Mutational analysis 、 Cancer research 、 COLD-PCR 、 In patient 、 Melting curve analysis 、 Diagnostic accuracy 、 Colorectal cancer 、 Epidermal growth factor receptor 、 KRAS 、 Medicine
摘要: Background KRAS mutational analysis is the standard of care prior to initiation treatments targeting epidermal growth factor receptor (EGFR) in patients with metastatic colorectal cancer. Sensitive methods are required reliably detect KRAS mutations tumor samples due admixture non-mutated cells. Many laboratories have implemented sensitive tests for mutations, but often require expensive instrumentation and reagents, parallel reactions, multiple steps, or opening PCR tubes.
biomedcentral.com
core.ac.uk
springer.com
sci-hub.se 参考文章(23)
Paul F. Engstrom, Juan Pablo Arnoletti, Al B. Benson, Yi-Jen Chen, Michael A. Choti, Harry S. Cooper, Anne Covey, Raza A. Dilawari, Dayna S. Early, Peter C. Enzinger, Marwan G. Fakih, James Fleshman, Charles Fuchs, Jean L. Grem, Krystyna Kiel, James A. Knol, Lucille A. Leong, Edward Lin, Mary F. Mulcahy, Sujata Rao, David P. Ryan, Leonard Saltz, David Shibata, John M. Skibber, Constantinos Sofocleous, James Thomas, Alan P. Venook, Christopher Willett, NCCN Clinical Practice Guidelines in Oncology: colon cancer. Journal of The National Comprehensive Cancer Network. ,vol. 7, pp. 778- 831 ,(2009) , 10.6004/JNCCN.2009.0056
Jin Li, G. Mike Makrigiorgos, COLD-PCR: a new platform for highly improved mutation detection in cancer and genetic testing Biochemical Society Transactions. ,vol. 37, pp. 427- 432 ,(2009) , 10.1042/BST0370427
Zhuang Zuo, Su S Chen, Pranil K Chandra, John M Galbincea, Matthew Soape, Steven Doan, Bedia A Barkoh, Hartmut Koeppen, L Jeffrey Medeiros, Rajyalakshmi Luthra, Application of COLD-PCR for improved detection of KRAS mutations in clinical samples Modern Pathology. ,vol. 22, pp. 1023- 1031 ,(2009) , 10.1038/MODPATHOL.2009.59
Shuji Ogino, Takako Kawasaki, Mohan Brahmandam, Liying Yan, Mami Cantor, Chungdak Namgyal, Mari Mino-Kenudson, Gregory Y. Lauwers, Massimo Loda, Charles S. Fuchs, Sensitive Sequencing Method for KRAS Mutation Detection by Pyrosequencing The Journal of Molecular Diagnostics. ,vol. 7, pp. 413- 421 ,(2005) , 10.1016/S1525-1578(10)60571-5
Lasse S. Kristensen, Iben L. Daugaard, Mariann Christensen, Stephen Hamilton-Dutoit, Henrik Hager, Lise Lotte Hansen, Increased sensitivity of KRAS mutation detection by high‐resolution melting analysis of COLD‐PCR products Human Mutation. ,vol. 31, pp. 1366- 1373 ,(2010) , 10.1002/HUMU.21358
Helena Linardou, Issa J. Dahabreh, Dimitrios Bafaloukos, Paris Kosmidis, Samuel Murray, Somatic EGFR mutations and efficacy of tyrosine kinase inhibitors in NSCLC Nature Reviews Clinical Oncology. ,vol. 6, pp. 352- 366 ,(2009) , 10.1038/NRCLINONC.2009.62
Ji-Dung Luo, Err-Cheng Chan, Chun-Liang Shih, Tai-Long Chen, Ying Liang, Tsann-Long Hwang, Chiuan-Chian Chiou, Detection of rare mutant K-ras DNA in a single-tube reaction using peptide nucleic acid as both PCR clamp and sensor probe. Nucleic Acids Research. ,vol. 34, ,(2006) , 10.1093/NAR/GNJ008
Takashi Kobunai, Toshiaki Watanabe, Yoko Yamamoto, Kiyoshi Eshima, The frequency of KRAS mutation detection in human colon carcinoma is influenced by the sensitivity of assay methodology: A comparison between direct sequencing and real-time PCR Biochemical and Biophysical Research Communications. ,vol. 395, pp. 158- 162 ,(2010) , 10.1016/J.BBRC.2010.03.167
Ji Eun Oh, Hee Sun Lim, Chang Hyeok An, Eun Goo Jeong, Ji Youn Han, Sug Hyung Lee, Nam Jin Yoo, Detection of Low-Level KRAS Mutations Using PNA-Mediated Asymmetric PCR Clamping and Melting Curve Analysis with Unlabeled Probes The Journal of Molecular Diagnostics. ,vol. 12, pp. 418- 424 ,(2010) , 10.2353/JMOLDX.2010.090146
Marina N. Nikiforova, Roy A. Lynch, Paul W. Biddinger, Erik K. Alexander, Gerald W. Dorn, Giovanni Tallini, Todd G. Kroll, Yuri E. Nikiforov, RASPoint Mutations and PAX8-PPARγ Rearrangement in Thyroid Tumors: Evidence for Distinct Molecular Pathways in Thyroid Follicular Carcinoma The Journal of Clinical Endocrinology & Metabolism. ,vol. 88, pp. 2318- 2326 ,(2003) , 10.1210/JC.2002-021907