Potential clinical significance of plasma-based KRAS mutation analysis using the COLD-PCR/TaqMan®-MGB probe genotyping method
作者： PEIJIA LIU , HONGYAN LIANG , LI XUE , CHUN YANG , YANG LIU
DOI: 10.3892/ETM.2012.566
关键词:
摘要: Despite the improved ability to detect mutations in recent years, tissue specimens cannot always be procured a clinical setting, particularly from patients with recurrence of tumors or metastasis. Therefore, aim this study was investigate whether plasma is able used for mutation analysis instead specimens. We collected 62 colorectal cancer (CRC) prior treatment. DNA extracted and matched tumor tissues were obtained. Mutations KRAS amplified sequenced by regular polymerase chain reaction (PCR) co-amplification at lower denaturation temperature (COLD)-PCR. Plasma gene on codon 12 (GGT>GAT) detected using nested COLD-PCR/TaqMan® -MGB probe. compared. found 13 (21.0%) (19.4%) tissues. The consistency between 75% (9/12), which indicated high correlation corresponding (P<0.001; index, k=0.649). Notably, four (6.5%) had no detectable primary tumors, three (4.8%) samples. Thus, correlate status CRC. Our provides evidence suggest that may as potential medium CRC COLD-PCR/TaqMan-MGB probe method.
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