Two-round coamplification at lower denaturation temperature-PCR (COLD-PCR)-based sanger sequencing identifies a novel spectrum of low-level mutations in lung adenocarcinoma.
作者： Jin Li , Coren A. Milbury , Cheng Li , G. Mike Makrigiorgos
DOI: 10.1002/HUMU.21112
关键词: Exon 、 Genetics 、 COLD-PCR 、 Missense mutation 、 Sanger sequencing 、 Biology 、 Polymerase chain reaction 、 Genetic heterogeneity 、 DNA Mutational Analysis 、 Frameshift mutation
摘要: … and sequencing of low-level TP53 mutations abundant at as … fast COLD-PCR followed by Sanger sequencing. We used this … also has limitations as it was designed to maximize mutation …
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