Studies on proteolytic thermoduric psychrotrophic bacteria in milk and fermented milk products.

作者: M. B. Gandhi , N. V. Mane

DOI:

关键词: Cold storageGram-positive bacteriaPasteurizationPsychrotrophic bacteriaFood spoilageFood scienceFood contaminantExiguobacteriumFermented milk productsBiology

摘要: Pasteurization is adopted for making milk free from borne organisms. However thermoduric psychrotrophic bacteria that are capable of surviving pasteurization temperature and able to grow in a cold environment (7 o C) may fermented products during refrigerated storage. Thus they represent significant spoilage problem the storage dairy products. They also produce heat resistant exoenzymes such as protease, lipase etc. which withstand function attack protein fats products, thereby producing defects sour milk, gassiness, rancidity, bitter Due activities psychrotrophs studies were undertaken on these For studying psychrotrophs, collected five different dairies Kolhapur region Maharashtra, India. Total psycrotrophic count seasonal variations determined. In all 36 isolates obtained, three gram positive identified Macrococcus equipercicus, Exiguobacterium sp., Arthrobacter sp. basis morphological, biochemical, physiological features well 16S rRNA sequencing. Proteolytic analyzed by qualitative quantitative methods. All proteolysis, whereas showed greater proteolytic activity than

参考文章(14)
J G Rola, H Rozanska, M Baranova, A Laukova, O Burdova, HYGIENE OF PASTEURIZED MILK DEPENDING ON PSYCHROTROPHIC MICROORGANISMS Bulletin of The Veterinary Institute in Pulawy. ,vol. 46, ,(2002)
R. R. MEER, J. BAKER, F. W. BODYFELT, M. W. GRIFFITHS, Psychrotrophic Bacillus spp. in Fluid Milk Products: A Review. Journal of Food Protection. ,vol. 54, pp. 969- 979 ,(1991) , 10.4315/0362-028X-54.12.969
G Kouker, K E Jaeger, Specific and sensitive plate assay for bacterial lipases. Applied and Environmental Microbiology. ,vol. 53, pp. 211- 213 ,(1987) , 10.1128/AEM.53.1.211-213.1987
D. W. Johnston, J. Bruce, Incidence of thermoduric psychrotrophs in milk produced in the west of Scotland Journal of Applied Microbiology. ,vol. 52, pp. 333- 337 ,(1982) , 10.1111/J.1365-2672.1982.TB05061.X
M.A. Cousin, E.H. Marth, Psychrotrophic Bacteria cause Changes in Stability of Milk to Coagulation by Rennet or Heat Journal of Dairy Science. ,vol. 60, pp. 1042- 1047 ,(1977) , 10.3168/JDS.S0022-0302(77)83986-6
HITTU MATTA, V PUNJ, Isolation and identification of lipolytic, psychrotrophic, spore forming bacteria from raw milk International Journal of Dairy Technology. ,vol. 52, pp. 59- 62 ,(1999) , 10.1111/J.1471-0307.1999.TB02072.X
K.K. Kwan, B.J. Skura, Identification of Proteolytic Pseudomonads Isolated from Raw Milk Journal of Dairy Science. ,vol. 68, pp. 1902- 1909 ,(1985) , 10.3168/JDS.S0022-0302(85)81048-1
T. Sørhaug, L. Stepaniak, Psychrotrophs and their enzymes in milk and dairy products: Quality aspects Trends in Food Science & Technology. ,vol. 8, pp. 35- 41 ,(1997) , 10.1016/S0924-2244(97)01006-6
Emanuele Smacchi, Patrick F Fox, Marco Gobbetti, Purification and characterization of two extracellular proteinases from Arthrobacter nicotianae 9458 Fems Microbiology Letters. ,vol. 170, pp. 327- 333 ,(1999) , 10.1111/J.1574-6968.1999.TB13391.X
F. Eliskases-Lechner, W. Ginzinger, The bacterial flora of surface-ripened cheeses with special regard to coryneforms Lait. ,vol. 75, pp. 571- 584 ,(1995) , 10.1051/LAIT:1995644