A Potential Mechanism of a Cationic Cyclopeptide for Enhancing Insulin Delivery across Caco-2 Cell Monolayers
作者： Mingming Chang , Xiaohui Li , Yuming Sun , Fang Cheng , Yueqing Li
关键词: Biophysics 、 Cell 、 Insulin 、 Caco-2 、 Tight junction 、 Monolayer 、 Endocytosis 、 Biochemistry 、 Immunostaining 、 Chemistry 、 Paracellular transport
摘要: Effective delivery of therapeutic biomolecules across biomembranes is a challenging topic. A cationic cyclopeptide named TD-34 (ACSSKKSKHCG) was reported to improve insulin effectively. Based on our previous work, we investigated the mechanism for enhancing Caco-2 cell monolayers. Transport studies insulin, and accompanied with were performed respectively using monolayers at different conditions. Transepithelial electrical resistance (TEER) value monitored 24 h immediately after beginning transport experiments. Moreover, tight junction protein (Claudin-1) localized by confocal immunofluorescence microscopy. Results showed alone attributable multiple routes including passive diffusion. When or without treated monolayers, TEER values decreased reversibly, it correlated reappearance proteins immunostaining assay. It concluded that (TD-34) had potential enhance paracellular loosening reversibly.
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