Generation of Functional Neurons from Feeder-Free, Keratinocyte-Derived Equine Induced Pluripotent Stem Cells
作者： Ruchi Sharma , Matthew Robert Livesey , David JA Wyllie , Christopher Proudfoot , Christopher Bruce Alexander Whitelaw
关键词: KOSR 、 Stem cell 、 Biology 、 SOX2 、 Homeobox protein NANOG 、 Immunology 、 Induced pluripotent stem cell 、 Rex1 、 Directed differentiation 、 Embryonic stem cell 、 Cell biology
摘要: Pluripotent stem cells (PSCs) offer unprecedented biomedical potential not only in relation to humans but also companion animals, particularly the horse. Despite this, attempts generate bona fide equine embryonic have been unsuccessful. A very limited number of induced PSC lines so far generated from fibroblasts their for directed differentiation into clinically relevant tissues has explored. In this study, we used retroviral vectors pluripotent (iPSCs) with comparatively high efficiency keratinocytes. Expression endogenous markers (OCT4, SOX2, LIN28, NANOG, DNMT3B, and REX1) was effectively restored these cells, which could form vivo several tissue derivatives three germ layers, including functional neurons, keratinized epithelium, cartilage, bone, muscle, respiratory gastric epithelia. Comparative analysis different reprogrammed cell revealed an association between t...
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