Inhibition and inactivation of bovine mammary and liver UDP-galactose-4-epimerases.
作者： C R Geren , L M Geren , K E Ebner
DOI: 10.1016/S0021-9258(18)71869-3
关键词: Biochemistry 、 Uridine diphosphate galactose 、 NAD+ kinase 、 Uracil nucleotide 、 Carbohydrate Epimerases 、 Chemistry 、 Uridine diphosphate glucose 、 Uridine 、 Enzyme 、 Glycerol-3-phosphate dehydrogenase 、 Molecular biology
摘要: Bovine liver and mammary UDP-galactose-4-epimerases were investigated with respect to various inhibitors inactivators. Uridine nucleotides NADH are potent Ki values in the low micromolar range. The NAD+/NADH ratio may be an important physiological control mechanism for it affects markedly activity of enzyme 50% inhibition occurring at a 20:1. In presence uridine binding epimerases is enhanced. Consequently, effect changes vivo would not immediately apparent as slow down displacement by NAD+. Neither nor galactose 1-phosphate inhibits purified enzymes previously reported impure enzyme. provide almost total protection against first order inactivation trypsin allow determination dissociation constants. NAD+ partially protects inactivation. Inactivation sulfhydryl reagents complex results indicate that least three groups modified before occurs. Partial occurs upon modification 2-hydroxy-5-nitrogenzyl bromide. Some this provided combination UDP.
sci-hub.st 参考文章(17)
Elizabeth S. Maxwell, The enzymic interconversion of uridine diphosphogalactose and uridine diphosphoglucose. Journal of Biological Chemistry. ,vol. 229, pp. 139- 151 ,(1957) , 10.1016/S0021-9258(18)70602-9
C R Geren, K E Ebner, Purification and characterization of UDP-galactose-4-epimerase from bovine tissues. Journal of Biological Chemistry. ,vol. 252, pp. 2082- 2088 ,(1977) , 10.1016/S0021-9258(18)71868-1
Richard Langer, Luis Glaser, Interaction of Nucleotides with Liver Uridine Diphosphate-glucose-4′-epimerase Journal of Biological Chemistry. ,vol. 249, pp. 1126- 1132 ,(1974) , 10.1016/S0021-9258(19)42951-7
Hanne Troedsson, Katherine Sanford, Elizabeth A. Robinson, Herman M. Kalckar, Metabolic inhibition of mammalian uridine diphosphate galactose 4-epimerase in cell cultures and in tumor cells. Journal of Biological Chemistry. ,vol. 241, pp. 2737- 2745 ,(1966) , 10.1016/S0021-9258(18)96526-9
K Weber, M Osborn, The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis Journal of Biological Chemistry. ,vol. 244, pp. 4406- 4412 ,(1969) , 10.1016/S0021-9258(18)94333-4
Steve C. Magee, Kurt E. Ebner, Inactivation of Soluble Bovine Milk Galactosyltransferase (Lactose Synthetase) by Sulfhydryl Reagents and Trypsin Journal of Biological Chemistry. ,vol. 249, pp. 6992- 6998 ,(1974) , 10.1016/S0021-9258(19)42156-X
P. Roy-Burman, S. Roy-Burman, D. W. Visser, Showdomycin, A New Nucleoside Antibiotic Cancer Research. ,vol. 28, pp. 1605- 1610 ,(1968)
Robert Cohn, Stanton Segal, Regulation of mammalian liver uridine diphosphogalactose 4-epimerase by pyrimidine nucleotides Biochimica et Biophysica Acta. ,vol. 222, pp. 533- 536 ,(1970) , 10.1016/0304-4165(70)90148-0
Providencia Rodríguez, Olga Bello, Karl Gaede, Purification and properties of thyroidal UDP-glucose-4-epimerase Archives of Biochemistry and Biophysics. ,vol. 143, pp. 579- 586 ,(1971) , 10.1016/0003-9861(71)90242-6
C.M. Tsai, N. Holmberg, K.E. Ebner, Purification, stabilization, and properties of bovine mammary UDP-galactose 4-epimerase Archives of Biochemistry and Biophysics. ,vol. 136, pp. 233- 244 ,(1970) , 10.1016/0003-9861(70)90347-4