Optimization of pre- treatments with Propidium Monoazide and PEMAX™ before real-time quantitative PCR for detection and quantification of viable Helicobacter pylori cells.
作者： María Antonia Ferrús , Jorge García-Hernández , Irene Hortelano , María Yolanda Moreno
DOI: 10.1016/J.MIMET.2021.106223
关键词: Real-time polymerase chain reaction 、 Incubation period 、 Cell 、 Biology 、 Helicobacter pylori 、 Dead cell 、 Pre treatment 、 Propidium monoazide 、 Molecular biology 、 Cell stress
摘要: … EMA and PMA, derived from ethidium bromide and propidium iodide respectively, are membrane-impermeant dyes that can penetrate only compromised membranes of non-viable cells…
sci-hub.st 参考文章(53)
Muhannad Sarem, Rodolfo Corti, Role of Helicobacter pylori coccoid forms in infection and recrudescence Gastroenterología y Hepatología. ,vol. 39, pp. 28- 35 ,(2016) , 10.1016/J.GASTRE.2015.12.005
Muhannad Sarem, Rodolfo Corti, Rol de las formas cocoides de Helicobacter pylori en la infección y la recrudescencia Gastroenterología y Hepatología. ,vol. 39, pp. 28- 35 ,(2016) , 10.1016/J.GASTROHEP.2015.04.009
Laura Moreno-Mesonero, Yolanda Moreno, José Luis Alonso, M. Antonia Ferrús, DVC-FISH and PMA-qPCR techniques to assess the survival of Helicobacter pylori inside Acanthamoeba castellanii. Research in Microbiology. ,vol. 167, pp. 29- 34 ,(2016) , 10.1016/J.RESMIC.2015.08.002
Sehee Nam, Soonbok Kwon, Min-jeong Kim, Jong-Chan Chae, Pil Jae Maeng, Jong-Geun Park, Gyu-Cheol Lee, Selective detection of viable Helicobacter pylori using ethidium monoazide or propidium monoazide in combination with real-time polymerase chain reaction Microbiology and Immunology. ,vol. 55, pp. 841- 846 ,(2011) , 10.1111/J.1348-0421.2011.00388.X
T Tsuchido, N Katsui, A Takeuchi, M Takano, I Shibasaki, Destruction of the outer membrane permeability barrier of Escherichia coli by heat treatment. Applied and Environmental Microbiology. ,vol. 50, pp. 298- 303 ,(1985) , 10.1128/AEM.50.2.298-303.1985
James D. Oliver, The Viable but Nonculturable State in Bacteria Journal of Microbiology. ,vol. 43, pp. 93- 100 ,(2005)
Gemma Agustí, Francesc Codony, Mariana Fittipaldi, Bárbara Adrados, Jordi Morató, Viability Determination of Helicobacter pylori Using Propidium Monoazide Quantitative PCR Helicobacter. ,vol. 15, pp. 473- 476 ,(2010) , 10.1111/J.1523-5378.2010.00794.X
Peyman Adibi, Farkhondeh Poursina, Tom Van de Wiele, Hajieh Ghasemian Safaei, Elham Hosseini, Helicobacter pylori in Iran: A systematic review on the association of genotypes and gastroduodenal diseases. Journal of Research in Medical Sciences. ,vol. 17, pp. 280- 292 ,(2012)
Sarah Huffer, Melinda E. Clark, Jonathan C. Ning, Harvey W. Blanch, Douglas S. Clark, Role of alcohols in growth, lipid composition, and membrane fluidity of yeasts, bacteria, and archaea. Applied and Environmental Microbiology. ,vol. 77, pp. 6400- 6408 ,(2011) , 10.1128/AEM.00694-11
Andre Hurst, Ashton Hughes, David L. Collins-Thompson, Bhagwandas G. Shah, Relationship between loss of magnesium and loss of salt tolerance after sublethal heating of Staphylococcus aureus. Canadian Journal of Microbiology. ,vol. 20, pp. 1153- 1158 ,(1974) , 10.1139/M74-178