Using phage display technology to obtain Crybodies active against non-target insects.

摘要: The insecticidal Cry toxins produced by Bacillus thuringiensis (Bt) are increasingly important in the biological control of insect pests and vectors human disease. Markets for Bt products transgenic plants expressing their driven specificity, safety move away from chemical agents. However, high specificity can also prove to be a limitation when there is no known toxin active against particular target. Novel activities discovered screening natural isolates or through modifications proteins. Here we demonstrate use λ-phage displaying Cry1Aa13 variants modified domain II loop 2 (Crybodies) select retargeted toxins. Through biopanning using gut tissue larvae non-target Aedes aegypti, isolated number phage further testing. Two overexpressed showed significant activity A. aegypti while another induced mortality at pupal stage. We present first report display identify novel toward insects distant taxonomic Orders establish this technology based on Crybodies as powerful tool developing tailor-made insecticides new target insects.