The interaction between Sendai virus and cell membranes *1A quantitative analysis of 125I-Sendai virus particles' association with human red blood cells

摘要: Intact Sendai virus particles were radiolabeled by the use of chloramine-T and Na 125I. The method described is reproducible, efficient appropriate for preparation large quantities biologically active with relatively high specific activity. Gel electrophoresis analysis revealed that approx. 50% total 125I incorporated in are associated two viral envelope glycoproteins, while remaining evenly distributed throughout other polypeptides. 125I-virus used to study some kinetic parameters interaction between human erythrocytes. Binding at 4 °C irreversible, non-cooperative exhibits a characteristic saturation curve. A maximum 1–2 × 103 bound per cell was derived from Non-radioactive native as well isolated glycophorin molecules competitively inhibit binding Incubation 37 virus-erythrocyte complex resulted release about 33% surrounding medium.