Long non-coding RNA growth arrest specific transcript 5 acts as a tumour suppressor in colorectal cancer by inhibiting interleukin-10 and vascular endothelial growth factor expression.
作者： Yuan Li , Yan Li , Shengkai Huang , Kun He , Mei Zhao
DOI: 10.18632/ONCOTARGET.14625
关键词: GAS5 、 Medicine 、 Immunology 、 Cancer 、 Cell growth 、 Vascular endothelial growth factor 、 Cancer research 、 Gene knockdown 、 Long non-coding RNA 、 NFKB1 、 Vascular endothelial growth factor A
摘要: Long non-coding RNAs (lncRNAs) are highly involved in diverse biological processes of human malignancies. The expression profile and underlying mechanism lncRNA growth arrest specific transcript 5 (GAS5) colorectal cancer (CRC) is poorly understood. In this study, we found that GAS5 was commonly downregulated CRC tissues, serum patients cell lines. Knockdown promoted proliferation colony formation, whereas overexpression produced the opposite result. We further demonstrated knockdown increased secretion interleukin-10 (IL-10) vascular endothelial factor (VEGF-A) via NF-κB Erk1/2 pathways. Neutralization IL-10 VEGF-A reduced tumour caused by knockdown. Moreover, showed a statistically significant correlation with mRNA levels tissues. illustrated markedly negatively correlated cytokine mouse model colitis-associated (CAC). These results delineate novel suppressing carcinogenesis. cytokines inhibited may provide targets for lncRNA-based therapies CRC.
impactjournals.com参考文章(45)
Annamaria Rapisarda, Giovanni Melillo, Role of the VEGF/VEGFR axis in cancer biology and therapy. Advances in Cancer Research. ,vol. 114, pp. 237- 267 ,(2012) , 10.1016/B978-0-12-386503-8.00006-5
Lucia Ricci-Vitiani, Giorgio Stassi, Francesca Di Gaudio, Monica Zerilli, Mariella Patti, Giuseppe Di Gesù, Diana Di Liberto, Ruggero De Maria, Matilde Todaro, Adamaria Florena, Thyroid cancer resistance to chemotherapeutic drugs via autocrine production of interleukin-4 and interleukin-10. Cancer Research. ,vol. 63, pp. 6784- 6790 ,(2003)
Jacques Ferlay, Isabelle Soerjomataram, Rajesh Dikshit, Sultan Eser, Colin Mathers, Marise Rebelo, Donald Maxwell Parkin, David Forman, Freddie Bray, Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. International Journal of Cancer. ,vol. 136, ,(2015) , 10.1002/IJC.29210
Xuefei Shi, Ming Sun, Hongbing Liu, Yanwen Yao, Rong Kong, Fangfang Chen, Yong Song, A critical role for the long non‐coding RNA GAS5 in proliferation and apoptosis in non‐small‐cell lung cancer Molecular Carcinogenesis. ,vol. 54, ,(2015) , 10.1002/MC.22120
Lisa M. Coussens, Zena Werb, Inflammation and cancer Nature. ,vol. 420, pp. 860- 867 ,(2002) , 10.1038/NATURE01322
Karin E. de Visser, Alexandra Eichten, Lisa M. Coussens, Paradoxical roles of the immune system during cancer development Nature Reviews Cancer. ,vol. 6, pp. 24- 37 ,(2006) , 10.1038/NRC1782
Clemens Neufert, Christoph Becker, Markus F Neurath, An inducible mouse model of colon carcinogenesis for the analysis of sporadic and inflammation-driven tumor progression Nature Protocols. ,vol. 2, pp. 1998- 2004 ,(2007) , 10.1038/NPROT.2007.279
Ziqiang Zhang, Z Zhu, K Watabe, X Zhang, C Bai, M Xu, F Wu, YY Mo, None, Negative regulation of lncRNA GAS5 by miR-21 Cell Death & Differentiation. ,vol. 20, pp. 1558- 1568 ,(2013) , 10.1038/CDD.2013.110
Boryana K. Popivanova, Kazuya Kitamura, Yu Wu, Toshikazu Kondo, Takashi Kagaya, Shiuchi Kaneko, Masanobu Oshima, Chifumi Fujii, Naofumi Mukaida, Blocking TNF-α in mice reduces colorectal carcinogenesis associated with chronic colitis Journal of Clinical Investigation. ,vol. 118, pp. 560- 570 ,(2008) , 10.1172/JCI32453
Dandan Yin, Xuezhi He, Erbao Zhang, Rong Kong, Wei De, Zhihong Zhang, Long noncoding RNA GAS5 affects cell proliferation and predicts a poor prognosis in patients with colorectal cancer Medical Oncology. ,vol. 31, pp. 253- ,(2014) , 10.1007/S12032-014-0253-8