Amphiphilic nanogel of enzymatically synthesized glycogen as an artificial molecular chaperone for effective protein refolding
作者： Shigeo Takeda , Haruko Takahashi , Shin-ichi Sawada , Yoshihiro Sasaki , Kazunari Akiyoshi
DOI: 10.1039/C3RA44572K
关键词: Nanogel 、 Amphiphile 、 Protein refolding 、 Biophysics 、 Cyclodextrin 、 Carbonic anhydrase 、 Chemistry 、 Dissociation (chemistry) 、 Biochemistry 、 Glycogen 、 General chemistry 、 General Chemical Engineering
摘要: Enzymatically synthesized glycogen (ESG) bearing hydrophobic groups were prepared to construct a new artificial molecular chaperone system. The radius of the amphiphilic ESG nanogel was approximately 15 nm. nanogels prevented irreversible aggregation carbonic anhydrase. Cyclodextrin induced release anhydrase in its active form via one-step mechanism without dissociation nanogels.
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