Efficacy of the antimicrobial peptide TP4 against Helicobacter pylori infection: in vitro membrane perturbation via micellization and in vivo suppression of host immune responses in a mouse model.

摘要: // Jayaram Lakshmaiah Narayana 1,2 , Han-Ning Huang 2 Chang-Jer Wu 3 and Jyh-Yih Chen 1 Doctoral Degree Program in Marine Biotechnology, Academia Sinica National Sun Yat-sen University, Kaohsiung, Taiwan Research Station, Institute of Cellular Orgasmic Biology, Sinica, Jiaushi, Ilan, Department Food Science, Ocean Keelung, Correspondence to: Chen, email: Wu, Keywords : Helicobacter pylori, antimicrobial peptide, tilapia piscidin 4 (TP4), micellization Received February 16, 2015 Accepted April 09, Published May 11, Abstract pylori infection is marked by a strong association with various gastric diseases, including gastritis, ulcers, cancer. Antibiotic treatment regimens have low success rates due to the rapid occurrence resistant H. strains, necessitating development novel anti- strategies. Here, we investigated therapeutic potential Tilapia Piscidin against multidrug pathogen based on its vitro vivo efficacy.TP4 inhibited growth both antibiotic-sensitive -resistant ( Cag A + Vac ) via membrane micelle formation, which led depolarization extravasation cellular constituents. During colonization tissue, maintains high T regulatorysubsets Th17/Treg ratio, results expression pro- anti-inflammatory cytokines. Treatment TP4 suppressed Treg subset populations inflammatory restored balance, resulted early clearance density recovery morphology. Toxicity studies demonstrated that has no adverse effects mice or rabbits. The this study indicate may be an effective safe monotherapeutic agent for infections.