microRNA-129-5p suppresses Adriamycin resistance in breast cancer by targeting SOX2.
作者： Huijuan Zeng , Lulu Wang , Jingjie Wang , Tao Chen , Hanjun Li
DOI: 10.1016/J.ABB.2018.05.018
关键词: IC50 、 Downregulation and upregulation 、 SOX2 、 Apoptosis 、 Breast cancer 、 microRNA 、 Wound healing 、 Cancer 、 Cancer research 、 Medicine
摘要: Abstract Adriamycin resistance is closely related to therapeutic efficacy in breast cancer patients and their prognosis. Increasing evidence has suggested that miRNA functions various types of cancer. microRNA-129-5p (miR-129-5p) been considered a tumor-suppressive several cancers, but its potential role not fully elucidate. By qRT-PCR assay, we revealed the expression miR-129-5p was significantly decreased tissues Adriamycin-resistant cells (MDA-MB-231/ADR, MCF-7/ADR). CCK-8, colony formation, wound healing, Transwell invasion, flow cytometric profiles were examined determine influence on in vitro. The upregulation IC50 concentration invasion promoted apoptosis MDA-MB-231/ADR presence Adriamycin, whereas Sex-Determining Region Y-Box 2 (SOX2) reversed these effects. A luciferase reporter assay confirmed binding 3′UTR SOX2. Collectively, it suppresses by directly targeting
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