Rejection criteria for the asymmetric carrier and their application to glucose transport in the human red blood cell

作者: B.L. Hankin , W.R. Lieb , W.D. Stein

DOI: 10.1016/0005-2736(72)90229-5

关键词: Carrier modelTetramerRed cell membraneRed blood cellGlucose transporterMembrane transportFacilitated diffusionSugar transportChemistryChromatographyBiophysicsCell biologyBiochemistry

摘要: Abstract 1. We have derived two criteria, either of which is sufficient to permit the rejection conventional, asymmetric carrier model for facilitated diffusion any given membrane transport system. These criteria involve only measurable parameters transport. 2. measured half-saturation concentration glucose at inner face human red cell 20 °C using infinite-cis procedure and found it be 2.8 mM, with a standard deviation 1.5 mM (eight observations). 3. Using this result previously determined experimental prameters, both our force us rule out as system blood cell. 4. Our results are consistent tetramer sugar

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