Kinetic parameters of glucose efflux from human red blood cells under zero-trans conditions

作者: S.J.D. Karlish , W.R. Lieb , D. Ram , W.D. Stein

DOI: 10.1016/0005-2736(72)90014-4

关键词: BiophysicsMoleculeMembraneZero (complex analysis)BiologyKinetic energyAnalytical chemistryCellGlucose transporterIsotonicEffluxCell biologyBiochemistry

摘要: Abstract 1. Efflux of glucose from human red blood cells under zero- trans conditions has been measured at 20°. 2. The values the K m and v max were found to be 25 ± 3 mM 139 11 mmoles/min per 1 isotonic cell water, respectively. 3. These observations indicate that conventional, symmetric carrier is not a valid model for transport in cells. 4. Our results are consistent, however, with recently proposed which occurs by internal transfer molecules across protein tetramers embedded plasma membrane.

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