The kinetic parameters of the monosaccharide transfer system of the human erythrocyte.

摘要: Abstract 1. The kinetics of sugar transfer across the human erythrocyte membrane are complex and require for their description measurement at least three independent parameters: KS (the carrier-substrate dissociation constant), r ratio rates loaded unloaded carrier) Vmax., equil. limiting rate when both sides saturated with permeant). 2. Methods described determining is analysed. half-saturation constant Km in terms 3. For glucose, 11 mM 25°, 20mM 13° 44mM 5°. (in μmoles per ml cell water min) decreases from 400 25% to 66 4. galactose, 95 25°. 5. At values 3–5 glucose entry egress 40–50 galactose entry. These data that r=3 or 4 if accord be achieved. 6. 8δ, approx. 1 while zero only one side other sugar-free) 20 min.