RNY-derived small RNAs as a signature of coronary artery disease.

摘要: Data from next generation sequencing technologies uncovered the existence of many classes small RNAs. Recent studies reported that RNAs are released by cells and can be detected in blood. In this report, we aimed to discover occurrence novel circulating coronary artery disease (CAD). We used high-throughput human mouse apoptotic primary macrophages, analyzed data empirical Bayes moderated t-statistics assess differential expression Benjamini Hochberg method control false discovery rate. Results were then confirmed Northern blot RT-qPCR foam two animal models for atherosclerosis, namely ApoE −/− Ldlr lines. Quantitative RT-PCR detect identified RNAs, RNY-derived was performed using sera 263 patients with CAD compared 514 matched healthy controls; Student t-test applied statistically differences. Associations clinical characteristics biological markers tested Spearman’s rank correlations, while multivariate logistic regressions test statistical association RNA levels CAD. Here, report that, macrophages stimulated pro-apoptotic or pro-atherogenic stimuli, Ro-associated non-coding called RNYs Y-RNAs, processed into (~24–34 nt) referred as small-RNYs (s-RNYs), including s-RNY1-5p RNY1. A significant upregulation s-RNY found aortic arches blood plasma mice serum (P <0.001). Biostatistical analysis revealed a positive hs-CRP ApoB levels; however, no interaction between either these relation status. Levels also independent statin fibrate therapies. Our results position relevant diagnostic biomarker atherosclerosis-related diseases. Measurement would valuable companion monitor cell apoptosis during atherosclerosis pathogenesis evaluate patient’s responsiveness future therapeutic strategies aiming attenuate advanced atherosclerotic lesions.