THE BIOLOGICAL IMPORTANCE OF U.V.‐INDUCED DNA‐PROTEIN CROSS‐LINKING IN VIVO AND ITS PROBABLE CHEMICAL MECHANISM*
作者： KENDRIC C. SMITH
DOI: 10.1111/J.1751-1097.1968.TB08048.X
关键词: Stereochemistry 、 Polynucleotide 、 In vivo 、 Protein dna 、 Chemistry
摘要: — The increased sensitivity of Escherichia coli to killing by u.v. while frozen and the changes in as a function temperature during irradiation have been correlated with production DN A-protein cross-links shown not be related thymine dimer production. These results provide yet another example where dimers make little contribution inactivation biological system (but does detract from importance other systems). We reported that uracil cysteine can combine form 5-S-cysteine, 6-hydrouracil when solution these two compounds is irradiated (2357 A). This led us offer this compound model for mechanism which DNA protein are cross–linked vivo. now extended our studies kinetics photochemical addition 35S-cysteine various polynucleotides DNA. Selected rate constants (μM cysteine/μM nucleotide involved /erg/mm2× 108) follows: The native heat-denaturated accounted summation appropriate polynucleotides. RNA shows biphasic response probably amount single-stranded regions present. Preliminary indicate tyrosine serine also add photochemically DNA. RESUMEN La pronunciada sensibilidad del al ser irradiado por luz cuando esta congelado y los cambios en la con temperatura durante radiacion han sido relacionados el aumento produccion de uniones ADN-proleina se ha demostrado no tener relacion formacion dimeros timina. Los resultados aqui presentados proveen otro ejemplo para ilustrar que timina practicamente contribyen inactivacion un sistema biologico (pero niega importancia biologica otros sistemas). Hemos reportado uracilo cisteina pueden combinarse formar 5-S-cisteina. 6-hidrouracilo una solucion estos dos componentes es irradiada (2537 Esto nos conduce presentar este compuesto explicar mecanismo cual ADN proteina unen En actualidad hemos extendido nuestros estudios cinetica adicion fotoquimica 35S-cisteina varios polinucleotidos ADN. Las constantes velocidad cisteina/μM nucleotido/erg/mm2× son las siguientes: Las nativo desnaturalizado calor obtenerse sumando correspondientes. Algunos preliminares indican tirosina senna tambien adicionan fotoquimicamente
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