Analysis of soil whole- and inner-microaggregate bacterial communities.

摘要: Although soil structure largely determines energy flows and the distribution composition of microhabitats, little is known about how microbial community influenced by structural characteristics organic matter compartmentalization dynamics. A UV irradiation-based procedure was developed to specifically isolate inner-microaggregate communities, thus providing means analyze these communities in relation their environment. Whole- fractions undisturbed soils reclaimed after disturbance surface coal mining were analyzed using 16S rDNA terminal restriction fragment polymorphism (T-RFLP) sequence analyses determine salient bacterial characteristics. We hypothesized that environments select for definable that, due sequestered environment, would not be significantly impacted disturbance. However, T-RFLP analysis indicated distinct differences between populations inner-microaggregates soils. While both found dominated Actinobacteria, contained only Actinobacteridae, while Rubrobacteridae predominate. Spatial stratification division-level lineages within microaggregates also evidenced, with Proteobacteria clones being prevalent libraries derived from whole microaggregates. The fractionation methods employed this study therefore represent a valuable tool defining relationships biodiversity structure.