A Multiplex Allele Specific Polymerase Chain Reaction (MAS-PCR) for the Detection of Factor V Leiden and Prothrombin G20210A.
作者： Morteza Bagheri , Isa Abdi Rad
DOI:
关键词: Population 、 Genotype 、 Polymerase chain reaction 、 Multiplex 、 Point mutation 、 Molecular biology 、 Genetics 、 Medicine 、 Allele 、 Factor V Leiden 、 Prothrombin G20210A
摘要: Introduction: In order to determine the frequencies of factor V Leiden and prothrombin G20210A point mutations in Iranian population with Azeri Turkish origin. Material methods: 120 unrelated individuals from general randomly selected were examined for using a multiplex allele specific polymerase chain reaction (MAS-PCR) assay. Outcomes: The frequency mutation was 2.08%, which means 5 chromosomes out 240 had mutation. distribution 20210 GG, GA, AA genotypes 20210A 37(92.5%), 3(7.5%), 0(0%) 3(3.75%) males 78(97.5%), 2(2.5%), 2(1.25%) females, respectively. Factor not found our tested group (zero chromosomes). Analysis observed studied groups indicates that there is no statistically significant difference between females males, regarding (p value>0.05). Conclusions: This first study its own kind this implies G1691A (R506Q, FV-Leiden) extremely low but more frequent group.
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