Development of a single‐chain variable fragment antibody‐based enzyme‐linked immunosorbent assay for determination of fumonisin B1 in corn samples
作者： Long Zou , Yang Xu , Yanping Li , Qinghua He , Bo Chen
DOI: 10.1002/JSFA.6505
关键词: Phage display 、 Phagemid 、 Chemistry 、 Chromatography 、 Fumonisin B1 、 Antibody 、 Overlap extension polymerase chain reaction 、 Mycotoxin 、 Single-chain variable fragment 、 Molecular biology 、 Monoclonal antibody
摘要: Abstract BACKGROUND Fumonisin B1 (FB1) is a cancer‐promoting mycotoxin produced by Fusarium species and one of the major food‐borne toxins in corn and corn products. The objective of this study was to produce a single‐chain variable fragment (scFv) antibody for determination of FB1 in corn samples. RESULTS Anti‐FB1 monoclonal antibodies were obtained via the hybridoma technique. Specific heavy‐and light‐chain variable fragments were amplified with degenerate primers and constructed into scFv antibody fragments by …
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