Determination of total fumonisins in corn by competitive direct enzyme-linked immunosorbent assay: Collaborative study

摘要: Fumonisins-mycotoxins produced by some Fusarium species-have been shown to be the causative agent of diseases in horses and other domesticated animals as well possible carcinogens humans. A collaborative study was conducted evaluate effectiveness a competitive direct enzyme-linked immunosorbent assay (CD-ELISA) for determination total fumonisins (B 1 , B 2 3 ) corn. The test portion extracted with methanol-water (7 + 3), filtered, diluted, tested on CD-ELISA. Naturally artificially contaminated corn portions were sent 13 collaborators United States. field prepared at different levels. Artificially spiked 1.0, 3.0, 5.0 mg/kg ). Average recoveries 120, 100, 90%, respectively. relative standard deviations repeatability ranged from 13.3 23.3% reproducibility 15.8 30.3% across all levels tested. HORRAT values, calculated each individual sample, 1.24 1.94. This method demonstrated acceptable intra- interlaboratory precision