Recovery of vancomycin-resistant gram-positive cocci from children.

摘要: A cross-sectional survey of vancomycin-resistant gram-positive cocci (VRGPC) in the feces children was initiated after several bacteremic infections with these organisms occurred at our hospital. selective medium consisting colistin-nalidixic acid agar, 5% sheep blood, vancomycin (5 mg/liter), and amphotericin B (8 mg/liter) developed to isolate VRGPC. single stool specimen submitted clinical microbiology laboratory from each 48 patients inoculated onto medium. Plates were incubated 35 degrees C carbon dioxide examined 24, 48, 72 h. Susceptibilities determined by broth microdilution. total 14 isolates 11 (22%) recovered. The density growth ranged a colony 2+. VRGPC identified as Leuconostoc lactis (n = 2), Lactobacillus confusus 4), Enterococcus species 5), Lactococcus 3). One strain recovered both blood one patients. MICs 4 micrograms/ml for isolates, 8 four more than 16 remaining eight isolates. All susceptible penicillin ampicillin. Only 1 had received prior treatment vancomycin. We conclude that low concentrations may be common gastrointestinal tracts children.