Weissella confusa: problems with identification of an opportunistic pathogen that has been found in fermented foods and proposed as a probiotic

作者: Marilynn R. Fairfax , Paul R. Lephart , Hossein Salimnia

DOI: 10.3389/FMICB.2014.00254

关键词: 16S ribosomal RNAProbioticWeissella confusaMicrobiologyFermentation in food processingOpportunistic pathogenBiologyOrganismIdentification (biology)Viridans streptococci

摘要: Weissella confusa is found in fermented foods and has been suggested as a probiotic, but also causes sepsis other serious infections humans animals. The incidence of human underestimated partly due to confusion with viridans streptococci difficulty making definitive identification, even if the organism recognized belong another genus, owing inability commercial systems identify it. We report our experiences identifying W. isolated from two immune-compromised patients, both whom developed this organism. Two gram positive MicroScan combination panels, could not because they did have their data bases, provide false identification. base RapID™ Str panel (Remel), which gave three incorrect, high probability results (≥ 95%). 16S rDNA sequencing identified isolates confusa. Maldi-Tof, performed by reference laboratories, correctly isolates.

参考文章(17)
H. Salimnia, D. Patel, P.R. Lephart, M.R. Fairfax, P.H. Chandrasekar, Listeria grayi : vancomycin‐resistant, gram‐positive rod causing bacteremia in a stem cell transplant recipient Transplant Infectious Disease. ,vol. 12, pp. 526- 528 ,(2010) , 10.1111/J.1399-3062.2010.00539.X
M Green, R M Wadowsky, K Barbadora, Recovery of vancomycin-resistant gram-positive cocci from children. Journal of Clinical Microbiology. ,vol. 28, pp. 484- 488 ,(1990) , 10.1128/JCM.28.3.484-488.1990
M-R Lee, Y-T Huang, C-H Liao, C-C Lai, P-I Lee, P-R Hsueh, None, Bacteraemia caused by Weissella confusa at a university hospital in Taiwan, 1997-2007 Clinical Microbiology and Infection. ,vol. 17, pp. 1226- 1231 ,(2011) , 10.1111/J.1469-0691.2010.03388.X
N.P. Harlan, R.R. Kempker, S.M. Parekh, E.M. Burd, D.T. Kuhar, Weissella confusa bacteremia in a liver transplant patient with hepatic artery thrombosis. Transplant Infectious Disease. ,vol. 13, pp. 290- 293 ,(2011) , 10.1111/J.1399-3062.2010.00579.X
H. Salimnia, G.J. Alangaden, R. Bharadwaj, T.M. Painter, P.H. Chandrasekar, M.R. Fairfax, Weissella confusa: an unexpected cause of vancomycin-resistant gram-positive bacteremia in immunocompromised hosts Transplant Infectious Disease. ,vol. 13, pp. 294- 298 ,(2011) , 10.1111/J.1399-3062.2010.00586.X
Kang Wook Lee, Ji Yeong Park, Hee Rok Jeong, Ho Jin Heo, Nam Soo Han, Jeong Hwan Kim, Probiotic properties of Weissella strains isolated from human faeces Anaerobe. ,vol. 18, pp. 96- 102 ,(2012) , 10.1016/J.ANAEROBE.2011.12.015
Vincenzina Fusco, Grazia M. Quero, Gaetano Stea, Maria Morea, Angelo Visconti, Novel PCR-based identification of Weissella confusa using an AFLP-derived marker. International Journal of Food Microbiology. ,vol. 145, pp. 437- 443 ,(2011) , 10.1016/J.IJFOODMICRO.2011.01.015
Funmilola A Ayeni, Borja Sánchez, Bolanle A Adeniyi, Clara G De los Reyes-Gavilan, Abelardo Margolles, Patricia Ruas-Madiedo, None, Evaluation of the functional potential of Weissella and Lactobacillus isolates obtained from Nigerian traditional fermented foods and cow's intestine. International Journal of Food Microbiology. ,vol. 147, pp. 97- 104 ,(2011) , 10.1016/J.IJFOODMICRO.2011.03.014