Cell-Specific Expression of the Mouse Glycoprotein Hormone α-Subunit Gene Requires Multiple Interacting DNA Elements in Transgenic Mice and Cultured Cells

摘要: The glycoprotein hormone alpha-subunit gene is expressed and differentially regulated in pituitary gonadotropes thyrotropes. Previous expression studies suggested that cell specificity may be by distinct DNA elements. We have identified an enhancer region between -4.6 -3.7 kb critical for high level both gonadotrope thyrotrope cells of transgenic mice. Fusion the to -341/+43 mouse promoter results appropriate transgene levels are similar observed with intact kb/+43 construct. Deletion sequences -341 -297 resuited a loss specificity, exhibited ectopic activation GH-, ACTH-, PRL-producing as well other peripheral tissues. Consistent these results, transient transfection demonstrated stimulated activity alpha-promoter alphaTSH alphaT3 cells, but it did not enhance significantly CV-1 cells. Removal allowed function heterologous Loss due required binding LIM homeoproteins or alpha-basal element 1. These data demonstrate requires participation proximal distal suggests from restricting anterior pituitary.