T lymphocyte-triggering factor of african trypanosomes is associated with the flagellar fraction of the cytoskeleton and represents a new family of proteins that are present in several divergent eukaryotes.

摘要: Abstract The trypanosome cytoskeleton consists almost entirely of microtubule-based structures. Although α- and β-tubulin from Trypanosoma brucei have been well characterized, much less is known about other cytoskeleton-associated proteins in trypanosomes. Using biochemical fractionation, we demonstrate here that T lymphocyte-triggering factor (TLTF) T. a component the detergent-resistant Ca2+-resistant fraction parasite cytoskeleton. This contains flagellar apparatus subset cytoskeletal protein complexes together function cell motility, cytokinesis, organelle inheritance. We also show TLTF-related genes are present several highly divergent eukaryotic organisms. corresponding not known, mammalian TLTF-like gene (GAS11; growtharrest-specific 11) up-regulated growth-arrested cells candidate tumor suppressor (Whitmore, S. A., Settasatian, C., Crawford, J., Lower, K. M., McCallum, B., Seshadri, R., Cornelisse, C. Moerland, E. W., Cleton-Jansen, A. Tipping, Mathew, G., Savnio, Savoia, Verlander, P., Auerbach, D., Van Berkel, Pronk, J. Doggett, N. Callen, D. F. (1998) Genomics 52, 325–331), suggestive role coordinating activities. Consistent with this possibility, human GAS11 144-amino acid domain co-localizes microtubules when fused to green fluorescent expressed cells. These findings suggest TLTF represents newly defined family, whose members contribute species as diverse protozoa mammals.