Ribosomal RNA cistrons in Euglena gracilis

作者: N.Steele Scott

DOI: 10.1016/0022-2836(73)90144-7

关键词: RNAEuglena gracilisRibosomeChloroplast DNARibosomal RNADNAMolecular biologyCytoplasmChloroplastBiology

摘要: Abstract Euglena gracilis chloroplasts contain about 12 fg DNA of average density 1.686 g cm −3 and 1.7 pg RNA. The large (1.1 × 10 6 mol. wt) small (0.56 ribosomal RNA components are coded for by separate cistrons, both which band at a 1.696 in CsCl gradient. About 6% the chloroplast codes rRNA indicating that there 240 cistrons each or three to six per genome. Similar studies with from cytoplasmic ribosomes indicate 1.716 , denser than main-band DNA, 1000 cell. Fractionation E. on gradients subsequent hybridization experiments, as well melting curves DNA-RNA hybrids, show does not anneal specifically either any dark-grown cell, contrast those results found some higher plants.

参考文章(18)
W.G. Flamm, H.E. Bond, H.E. Burr, Density-Gradient centrifugation of DNA in a fixed-angle rotor: A higher order of resolution Biochimica et Biophysica Acta. ,vol. 129, pp. 310- 317 ,(1966) , 10.1016/0005-2787(66)90373-X
E.S. Kempner, J.H. Miller, The molecular biology of Euglena gracilis. I. Growth conditions and cellular composition. Biochimica et Biophysica Acta. ,vol. 104, pp. 11- 17 ,(1965) , 10.1016/0304-4165(65)90214-X
Erhard Stutz, The kinetic complexity of Euglena gracilis chloroplasts DNA FEBS Letters. ,vol. 8, pp. 25- 28 ,(1970) , 10.1016/0014-5793(70)80216-2
STUART M. RIDLEY, RACHEL M. LEECH, Division of Chloroplasts in an Artificial Environment Nature. ,vol. 227, pp. 463- 465 ,(1970) , 10.1038/227463A0
Erhard Stutz, Jobst P. Vandrey, Ribosomal DNA satellite of Euglena gracilis chloroplast DNA FEBS Letters. ,vol. 17, pp. 277- 280 ,(1971) , 10.1016/0014-5793(71)80164-3
George Brawerman, J.M. Eisenstadt, Deoxyribonucleic acid from the chloroplasts of Euglena gracilis Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects. ,vol. 91, pp. 477- 485 ,(1964) , 10.1016/0926-6550(64)90077-5
J. Marmur, A procedure for the isolation of deoxyribonucleic acid from micro-organisms Journal of Molecular Biology. ,vol. 3, pp. 208- IN1 ,(1961) , 10.1016/S0022-2836(61)80047-8
N.Steele Scott, Rana Munns, Robert M. Smillie, Chloroplast and cytoplasmic ribosomes in Euglena gracilis FEBS Letters. ,vol. 10, pp. 149- 152 ,(1970) , 10.1016/0014-5793(70)80439-2
P. Detchon, J.V. Possingham, Ribosomal-RNA distribution during leaf development in spinach Phytochemistry. ,vol. 11, pp. 943- 947 ,(1972) , 10.1016/S0031-9422(00)88437-X