Vectorial secretion of extracellular matrix proteins, matrix-degrading proteinases, and tissue inhibitor of metalloproteinases by endothelial cells
作者： E N Unemori , K S Bouhana , Z Werb
DOI: 10.1016/S0021-9258(19)40250-0
关键词: Endothelial stem cell 、 Biology 、 Fibronectin 、 Secretion 、 Matrix metalloproteinase 、 Extracellular matrix 、 Matrix (biology) 、 Laminin 、 Cell culture 、 Molecular biology
摘要: In a study of the vectorial secretion proteins by bovine aortic arch endothelial cells, we found that extracellular matrix macromolecules collagen and fibronectin as well several matrix-degrading metalloproteinases were secreted selectively in basal direction. contrast, tissue inhibitor showed only weak preference for Three at 18-35 kDa with apically, counter to approximately 70% total protein. As expected, rabbit synovial fibroblasts, which used control, proteins, including collagen, gelatin-degrading proteinases, casein-degrading equally apical directions. The fibronectin, gelatinases, cells suggests structural functional polarity these is manifested, part, level polarized matrix-related proteins.
sci-hub.st 参考文章(37)
G Fastelli, L A Liotta, S Garbisa, A Negro, M Naturale, D Daga-Gordini, M Ballin, G Semenzato, Transient expression of type IV collagenolytic metalloproteinase by human mononuclear phagocytes. Journal of Biological Chemistry. ,vol. 261, pp. 2369- 2375 ,(1986) , 10.1016/S0021-9258(17)35946-X
T Salo, L A Liotta, K Tryggvason, Purification and characterization of a murine basement membrane collagen-degrading enzyme secreted by metastatic tumor cells. Journal of Biological Chemistry. ,vol. 258, pp. 3058- 3063 ,(1983) , 10.1016/S0021-9258(18)32829-1
D. M. Weir, Handbook of experimental immunology ,(1967)
H G Welgus, G P Stricklin, Human skin fibroblast collagenase inhibitor. Comparative studies in human connective tissues, serum, and amniotic fluid. Journal of Biological Chemistry. ,vol. 258, pp. 12259- 12264 ,(1983) , 10.1016/S0021-9258(17)44167-6
Z Werb, M J Banda, G S Herron, K Dwyer, Secretion of metalloproteinases by stimulated capillary endothelial cells. I. Production of procollagenase and prostromelysin exceeds expression of proteolytic activity. Journal of Biological Chemistry. ,vol. 261, pp. 2810- 2813 ,(1986) , 10.1016/S0021-9258(17)35859-3
M S Hibbs, K A Hasty, J M Seyer, A H Kang, C L Mainardi, Biochemical and immunological characterization of the secreted forms of human neutrophil gelatinase. Journal of Biological Chemistry. ,vol. 260, pp. 2493- 2500 ,(1985) , 10.1016/S0021-9258(18)89580-1
I E Collier, S M Wilhelm, A Z Eisen, B L Marmer, G A Grant, J L Seltzer, A Kronberger, C S He, E A Bauer, G I Goldberg, H-ras oncogene-transformed human bronchial epithelial cells (TBE-1) secrete a single metalloprotease capable of degrading basement membrane collagen. Journal of Biological Chemistry. ,vol. 263, pp. 6579- 6587 ,(1988) , 10.1016/S0021-9258(18)68680-6
Z Werb, J Gavrilovic, M J Banda, G S Herron, E J Clark, Secretion of metalloproteinases by stimulated capillary endothelial cells. II. Expression of collagenase and stromelysin activities is regulated by endogenous inhibitors. Journal of Biological Chemistry. ,vol. 261, pp. 2814- 2818 ,(1986) , 10.1016/S0021-9258(17)35860-X
Scheffer C. G. TSENG, Naphtali SAVION, Robert STERN, Denis GOSPODAROWICZ, Fibroblast Growth Factor Modulates Synthesis of Collagen in Cultured Vascular Endothelial Cells FEBS Journal. ,vol. 122, pp. 355- 360 ,(1982) , 10.1111/J.1432-1033.1982.TB05888.X
R Montesano, A Mossaz, J E Ryser, L Orci, P Vassalli, Leukocyte interleukins induce cultured endothelial cells to produce a highly organized, glycosaminoglycan-rich pericellular matrix. Journal of Cell Biology. ,vol. 99, pp. 1706- 1715 ,(1984) , 10.1083/JCB.99.5.1706